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goat anti gal 3  (R&D Systems)


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    R&D Systems goat anti gal 3
    Goat Anti Gal 3, supplied by R&D Systems, used in various techniques. Bioz Stars score: 94/100, based on 73 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/goat+anti+mouse+gal+3/Human%2FMouse%2FRat+Galectin-3+Antibody/pm35977543-323-5-8
    Average 94 stars, based on 73 article reviews
    goat anti gal 3 - by Bioz Stars, 2026-09
    94/100 stars

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    Related Articles

    Incubation:

    Article Title: The Diagnostic and Prognostic Value of Plasma Galectin 3 in HFrEF Related to the Etiology of Heart Failure
    Article Snippet: For Gal-3 immunofluorescent staining, after dewaxed, heat-induced antigen retrieval and permeabilization were carried out (with 10 mM of Na-citrate buffer containing 0.05% Tween 20; pH 6.0; 95°C for 25 min) followed by blocking with DAKO Protein Block (X0909, Agilent, 1 h at room temperature). .. Sections were incubated with primary goat anti-mouse Gal-3 (1:100, AF1197, R&D Systems) overnight at 4°C, then they were incubated with the secondary antibody, Alexa Fluor 594 donkey anti-goat IgG (1:200, A11058, Invitrogen by Thermo Fisher Scientific). .. The cardiomyocyte boundary was revealed by wheat-germ-agglutinin FITC staining (1:80, FL-1021, Vector Labs, 1 h at room temperature).

    Article Title: The Prognostic Value of Plasma Galectin 3 in HFrEF Depends on the Etiology of Heart Failure: a Cohort Study and Animal Experiment
    Article Snippet: For Gal-3 immuno uorescent staining, after samples had been dewaxed, heat-induced antigen retrieval and permeabilization were carried out (with 10 mM of Na-citrate buffer containing 0.05% Tween 20; pH 6.0; 95°C for 25 min) followed by blocking with DAKO Protein Block (X0909, Agilent, 1 h at room temperature). .. Sections were incubated with primary goat anti-mouse Gal-3 (1:100, AF1197, R&D Systems) overnight at 4°C, after which they were incubated with the secondary antibody, Alexa Fluor 594 donkey anti-goat IgG (1:200, A11058, Invitrogen by Thermo Fisher Scienti c). .. The cardiomyocyte boundary was revealed by wheat-germ-agglutinin FITC staining (1:80, FL-1021, Vector Labs, 1 h at room temperature).



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    Surface-associated glycoproteins and gamma-glutamyl transferase on the seminal prostasomes of normozoospermic men: influence of detergent treatment. Prostasomes from human seminal plasma of normozoospermic men (sPro-N) were subjected to Triton X-100 (TX-100) treatment followed by gel filtration on a Sephadex G-200 column. Reference elution profiles of native sPro-N (eluted at void volume) were shown for comparison. Elution of (a) concanavalin A-reactive glycans (Con A-R) and (b) wheat germ agglutinin-reactive glycans (WGA-R). (c) Elution of GGT. (d) Distribution of tetraspanins (CD63, CD9, and CD81) and <t>gal-3</t> (indicated in panel c) was monitored by measuring the immunoreactivity of dot blot-immobilized fractions. The presence of TX-100 in eluted fractions caused spilled appearance of dots and background staining. A450: absorbance at 450 nm; GGT: gamma-glutamyl transferase activity expressed in U/L, unit per liter; gal-3: galectin 3; F: fraction.
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    Surface-associated glycoproteins and gamma-glutamyl transferase on the seminal prostasomes of normozoospermic men: influence of detergent treatment. Prostasomes from human seminal plasma of normozoospermic men (sPro-N) were subjected to Triton X-100 (TX-100) treatment followed by gel filtration on a Sephadex G-200 column. Reference elution profiles of native sPro-N (eluted at void volume) were shown for comparison. Elution of (a) concanavalin A-reactive glycans (Con A-R) and (b) wheat germ agglutinin-reactive glycans (WGA-R). (c) Elution of GGT. (d) Distribution of tetraspanins (CD63, CD9, and CD81) and <t>gal-3</t> (indicated in panel c) was monitored by measuring the immunoreactivity of dot blot-immobilized fractions. The presence of TX-100 in eluted fractions caused spilled appearance of dots and background staining. A450: absorbance at 450 nm; GGT: gamma-glutamyl transferase activity expressed in U/L, unit per liter; gal-3: galectin 3; F: fraction.
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    Surface-associated glycoproteins and gamma-glutamyl transferase on the seminal prostasomes of normozoospermic men: influence of detergent treatment. Prostasomes from human seminal plasma of normozoospermic men (sPro-N) were subjected to Triton X-100 (TX-100) treatment followed by gel filtration on a Sephadex G-200 column. Reference elution profiles of native sPro-N (eluted at void volume) were shown for comparison. Elution of (a) concanavalin A-reactive glycans (Con A-R) and (b) wheat germ agglutinin-reactive glycans (WGA-R). (c) Elution of GGT. (d) Distribution of tetraspanins (CD63, CD9, and CD81) and <t>gal-3</t> (indicated in panel c) was monitored by measuring the immunoreactivity of dot blot-immobilized fractions. The presence of TX-100 in eluted fractions caused spilled appearance of dots and background staining. A450: absorbance at 450 nm; GGT: gamma-glutamyl transferase activity expressed in U/L, unit per liter; gal-3: galectin 3; F: fraction.
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    Surface-associated glycoproteins and gamma-glutamyl transferase on the seminal prostasomes of normozoospermic men: influence of detergent treatment. Prostasomes from human seminal plasma of normozoospermic men (sPro-N) were subjected to Triton X-100 (TX-100) treatment followed by gel filtration on a Sephadex G-200 column. Reference elution profiles of native sPro-N (eluted at void volume) were shown for comparison. Elution of (a) concanavalin A-reactive glycans (Con A-R) and (b) wheat germ agglutinin-reactive glycans (WGA-R). (c) Elution of GGT. (d) Distribution of tetraspanins (CD63, CD9, and CD81) and gal-3 (indicated in panel c) was monitored by measuring the immunoreactivity of dot blot-immobilized fractions. The presence of TX-100 in eluted fractions caused spilled appearance of dots and background staining. A450: absorbance at 450 nm; GGT: gamma-glutamyl transferase activity expressed in U/L, unit per liter; gal-3: galectin 3; F: fraction.

    Journal: Upsala Journal of Medical Sciences

    Article Title: Assembly of tetraspanins, galectin-3, and distinct N-glycans defines the solubilization signature of seminal prostasomes from normozoospermic and oligozoospermic men

    doi: 10.48101/ujms.v126.7673

    Figure Lengend Snippet: Surface-associated glycoproteins and gamma-glutamyl transferase on the seminal prostasomes of normozoospermic men: influence of detergent treatment. Prostasomes from human seminal plasma of normozoospermic men (sPro-N) were subjected to Triton X-100 (TX-100) treatment followed by gel filtration on a Sephadex G-200 column. Reference elution profiles of native sPro-N (eluted at void volume) were shown for comparison. Elution of (a) concanavalin A-reactive glycans (Con A-R) and (b) wheat germ agglutinin-reactive glycans (WGA-R). (c) Elution of GGT. (d) Distribution of tetraspanins (CD63, CD9, and CD81) and gal-3 (indicated in panel c) was monitored by measuring the immunoreactivity of dot blot-immobilized fractions. The presence of TX-100 in eluted fractions caused spilled appearance of dots and background staining. A450: absorbance at 450 nm; GGT: gamma-glutamyl transferase activity expressed in U/L, unit per liter; gal-3: galectin 3; F: fraction.

    Article Snippet: Monoclonal anti-CD63 antibody (clone TS63) was from Abcam (Cambridge, UK), monoclonal anti-CD81 (clone M38) and monoclonal anti-CD9 (clone MEM-61) were from Invitrogen by Thermo Fisher Scientific (Carlsbad, CA, USA), and biotinylated goat anti-galectin-3 (gal-3) antibodies were from R&D Systems (Minneapolis, USA).

    Techniques: Filtration, Comparison, Dot Blot, Staining, Activity Assay

    Surface-associated glycoproteins and gamma-glutamyl transferase on seminal prostasomes of oligozoospermic men: influence of detergent treatment. Prostasomes from human seminal plasma of oligozoospermic men (sPro-O) were subjected to Triton X-100 (TX-100) treatment followed by gel filtration on a Sephadex G-200 column. Reference elution profiles of native sPro-O from Sephadex G-200 column (eluted at void volume) were shown for comparison. Elution of (a) concanavalin A-reactive glycans (Con A-R) and (b) wheat germ agglutinin-reactive glycans (WGA-R). (c) Elution of GGT. (d) Distribution of tetraspanins (CD63, CD9, and CD81) and gal-3 (indicated in panel c) was monitored by measuring the immunoreactivity of dot blot-immobilized fractions. The presence of TX-100 in eluted fractions caused spilled appearance of dots and background staining. Barely detectable CD9- and gal-3-immunoreactivity is indicated by circle. A450, absorbance at 450 nm; GGT, gamma-glutamyl transferase activity expressed in U/L, unit per liter; gal-3, galectin 3; F, fraction.

    Journal: Upsala Journal of Medical Sciences

    Article Title: Assembly of tetraspanins, galectin-3, and distinct N-glycans defines the solubilization signature of seminal prostasomes from normozoospermic and oligozoospermic men

    doi: 10.48101/ujms.v126.7673

    Figure Lengend Snippet: Surface-associated glycoproteins and gamma-glutamyl transferase on seminal prostasomes of oligozoospermic men: influence of detergent treatment. Prostasomes from human seminal plasma of oligozoospermic men (sPro-O) were subjected to Triton X-100 (TX-100) treatment followed by gel filtration on a Sephadex G-200 column. Reference elution profiles of native sPro-O from Sephadex G-200 column (eluted at void volume) were shown for comparison. Elution of (a) concanavalin A-reactive glycans (Con A-R) and (b) wheat germ agglutinin-reactive glycans (WGA-R). (c) Elution of GGT. (d) Distribution of tetraspanins (CD63, CD9, and CD81) and gal-3 (indicated in panel c) was monitored by measuring the immunoreactivity of dot blot-immobilized fractions. The presence of TX-100 in eluted fractions caused spilled appearance of dots and background staining. Barely detectable CD9- and gal-3-immunoreactivity is indicated by circle. A450, absorbance at 450 nm; GGT, gamma-glutamyl transferase activity expressed in U/L, unit per liter; gal-3, galectin 3; F, fraction.

    Article Snippet: Monoclonal anti-CD63 antibody (clone TS63) was from Abcam (Cambridge, UK), monoclonal anti-CD81 (clone M38) and monoclonal anti-CD9 (clone MEM-61) were from Invitrogen by Thermo Fisher Scientific (Carlsbad, CA, USA), and biotinylated goat anti-galectin-3 (gal-3) antibodies were from R&D Systems (Minneapolis, USA).

    Techniques: Filtration, Comparison, Dot Blot, Staining, Activity Assay